BP701T-Instrumental Method of Analysis

Max Marks: 75
Max Time: 3 hours

Instructions to candidate

1. Do not write anything on question paper except Seat No.
2. Graph or diagram should be drawn with the black ink
3. All questions are compulsory.
4. Figures to right indicate full marks.
5. Students should note, no supplement will be provided.

Q1 Answer All Questions (20 Marks)

1. When absorption maxima (λmax) of a compound is shifted towards longer wavelength, It is called as

a) Bathochromic shift
b) Hypsochromic shift
c) Hyperchromic shift
d) Hypochromic shift

2. Stationary phase is non polar and mobile phase is polar is called as

a) Bonded phase
b) Normal phase
c) Reverse phase
d) Non-bonded

3. Action of opposite ions is utilized in-

a) Affinity chromatography
b) lon exchange chromatography
c) Size exclusion chromatography
d) HPLC

4. In chromatography, the mobile phase can be made of?

a) Solid or liquid
b) Liquid or gas
c) Gas only
d) Liquid only

5. Which of the following cannot be used as an adsorbent in column adsorption chromatography?

a) Magnesium oxide
b) Silica gel
c) Activated alumina
d) Potassium permanganate

6. A molecule can only absorb IR radiation when its absorption causes a change in its-

a) Conductivity
b) Electric dipole
c) Polarity
d) Potential difference

7. The most commonly used mulling reagent in IR is

a) Nujol
b) KCl
c) HCl
d) Iodine

8. Which technique separates charged particles using electric field?

a) Hydrolysis
b) Electrophoresis
c) Polarity
d) protein denaturing

9. For amino acid detection _____ is used as a visualizing reagents

a) Dragendroff’s reagent
b) 3,5-dinitrobenzoic acid
c) Ninhydrin in acetone
d) Fehling’s reagent

10. The first step in preparation of affinity chromatography is

a) Ligand attachment to matrix
b) coupling of aromatic amines to matrix
c) Activation process
d) precipitation

11. The sample cell is made up of _____ in UV spectroscopy

a) stainless steel
b) plastic
c) quartz
d) Glass

12. Lambert’s law is concerned with

a) Concentration
b) Thickness of medium
c) Volume
d) Composition

13. Which of the following is the example of pyroelectric material used in pyroelectric tranducers in IR spectroscopy

a) Lead sulphite
b) Indium antimonite
c) Bismuth-antimony
d) Triglycine sulphate

14. The source of continuous radition commonly employed in fluorescence spectroscopy is  

a) Hydrogen or deuterium discharge lamp
b) Xenon arc lamp
c) Hallow cathode lamp
d) Tungsten lamp

15. Derivatization technique in HPLC are intended to enhance

a) Molecular weight
b) Reversibility
c) Reproducibility
d) Detectability

16. Affinity chromatography can not be used for the –

a) Separation of proteins according to their isoelectric points
b) Purification of compounds from a complex mixtures
c) Study of enzyme-substrate interaction
d) Concentration of a compounds

17. Vaccum UV region generally lies between in which of the following range?

a) Below 200nm
b) 200-400nm
c) 400-800nm
d) 300-500nm

18. Which of the following bending vibrations takes place in different planes

a) Asymmetric stretching
b) Rocking
c) Scissoring
d) Twisting

19. The size of thin layer of adsorbent is about

a) 0.1mm
b) 0.2mm
c) 0.3mm
d) 0.4mm

20. The intensity of the transmitted light is usually measure at which angle?

a) 90º
b) 45º
c) 60º
d) 180º

Q2 Attempt any two of the following (20 Marks)

1. Define Fluorescence and Phosphorescence? Explain the various factors affecting on Fluorescence intensity in detail.
2. Explain in detail principle, instrumentation and applications of Double beam UV-Spectrophotometer.
3. Describe the principle, instrumentation of HPLC along with their applications.

Q3 Attempt any seven of the following (35 Marks)

1. What is the principle of Paper chromatography? Explain the various steps
   involved in Paper chromatography.
2. Comment on type of electrons and electronic transitions involved in UV-Spectroscopy.
3. Explain the various modes of vibration and sampling techniques used in IR
   Spectroscopy.
4. Discuss the principle and applications of AAS. Add a note on Hollow cathode lamp.
5. Give the Principle of Nephelometry? Explain in brief instrumentation of Nephelometer
6. Write the principle and development techniques used in Thin layer chromatography.
7. State Beer’s and Lambert’s law. Add a note on Deviations of Beer’s law.
8. Write a note on Gel chromatography
9. Elaborate instrumentation and applications of Gas chromatography.